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Bowtie 2 is an ultrafast and memory-efficient tool for aligning sequencing reads to long reference sequences. It is particularly good at aligning reads of about 50 up to 100s or 1,000s of characters, and particularly good at aligning to relatively long (e.g. mammalian) genomes. Bowtie 2 indexes the genome with an FM Index to keep its memory footprint small: for the human genome, its memory footprint is typically around 3.2 GB. Bowtie 2 supports gapped, local, and paired-end alignment modes.


panX is a software package for comprehensive analysis, interactive visualization and dynamic exploration of bacterial pan-genomes. The analysis pipeline is based on DIAMOND, MCL and phylogeny-aware post-processing.


PGAweb is a webserver for bacterial pan-genome analysis. It had integrated our previous published stand-alone tools PGAP, PanGP and PGAP-X into one server and simplifies the analysis of pan-genome.

MetaPhlAn 3.0

MetaPhlAn is a computational tool for profiling the composition of microbial communities (Bacteria, Archaea and Eukaryotes) from metagenomic shotgun sequencing data (i.e. not 16S) with species-level. With the StrainPhlAn module, it is now possible to perform accurate strain-level microbial profiling.


QIIME 2 is a powerful, extensible, and decentralized microbiome analysis package with a focus on data and analysis transparency. QIIME 2 enables researchers to start an analysis with raw DNA sequence data and finish with publication-quality figures and statistical results.

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